研究方向:鱼类基因组学

金沙8888js官方教授，博士生导师，水产种质资源发掘和利用教育部重点实验室主任。中国科学院大学博士，在美国宾夕法尼亚大学从事博士后研究工作期间，开发了数种国际先进的高通量单细胞基因组学技术，不仅能精确解析细胞的异质性，而且能在极少量的生物材料上分析遗传和表观遗传的调控规律。从事单细胞基因组学新技术开发和应用的研究，开发了多种国际领先的新型高通量单细胞分析技术，取得了多项创新研究成果。在 Nature Methods、 Molecular Cell、Nucleic AcidsResearch、Genes & Development 等国际著名期刊上发表多篇论著。入选 2019 年国家级人才项目，任国际干细胞研究学会会员。

01 代表性论文

[1] Kwon, D. Y., Xu, B., Hu, P., Zhao, Y.-T., Beagan, J. A., Nofziger, J. H., Cui, Y., Phillips-Cremins, J. E., Blendy, J. A., Wu, H., & Zhou, Z. (2022). Neuronal Yin Yang1 in the prefrontal cortex regulates transcriptional and behavioral responses to chronic stress in mice. Nature Communications, 13(1), 55.

[2] Fabyanic E*, Hu P*, Qiu Q, Wang T, Berrios KN, Flournoy J, Connolly DR, Zhou Z, Kohil RM, Wu H (2021) Quantitative single cell 5hmC sequencing reveals non-canonical gene regulation by non-CG hydroxymethylation. Biorxiv, doi: https://doi.org/10.1101/2021.03.23.434325.

[3] Kwon DY, Xu B,Hu P, Zhao YT, Beagan JA, Nofziger JH, Cui Y, Zaitseva D, Phillips-Cremins JE, Wu H, Zhou Z (2021) Stress-induced epigenetic regulation of transcription in neocortical excitatory neurons drives depression-like behavior. Nature Communications (Accepted), Biorxiv, link doi:https://doi.org/10.1101/2020.07.06.190280.

[4] Qiu Q*, Hu P*(共同第一), Qiu X, Govek KW, Camara PG, Wu H (2020) Massively parallel and time-resolved single-cell RNA sequencing with scNT-Seq. Nature Methods, 17(10): 991-1001.

[5] Ho Y*, Hu P*(共同第一), Peel MT, Chen S, Camara PG, Epstein DJ, Wu H, Liebhaber SA (2020) Single cell transcriptomic analysis of the adult mouse pituitary reveals a novel multi-hormone cell cluster and physiologic demand-induced lineage plasticity. Protein & Cell: 1-19.

[6] Hu P*, Liu J*, Zhao J*, Wilkins BJ, Lupino K, Wu H, Pei L (2018) Single-nucleus transcriptomic survey of cell diversity and functional maturation in postnatal mammalian hearts. Genes & Development 32: 1344-1357.

[7] Schutsky EK, DeNizio JE, Hu P, Liu MY, Nabel CS, Fabyanic EB, Hwang Y, Bushman FD, Wu H, Kohli RM (2018) Nondestructive, base-resolution sequencing of 5-hydroxymethylcytosine using a DNA deaminase. Nature Biotechnology 36: 1083.

[8] Hu P*, Fabyanic E*, Kwon DY, Tang S, Zhou Z, Wu H (2017) Dissecting Cell-Type Composition and Activity-Dependent Transcriptional State in Mammalian Brains by Massively Parallel Single-Nucleus RNA-Seq. Molecular Cell 68: 1006-1015.e1007. 

[9] Hu P, Liu M, Liu Y, Wang J, Zhang D, Niu H, Jiang S, Wang J, Zhang D, Han B, Xu Q, Chen L (2016) Transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish.Scientific Reports6: 28952.

[10] Hu P, Liu M, Zhang D, Wang J, Niu H, Liu Y, Wu Z, Han B, Zhai W, Shen Y, Chen L (2015) Global identification of the genetic networks and cis-regulatory elements of the cold response in zebrafish. Nucleic Acids Research 43: 9198-9213.

[11] Zhang D*, Yu M*,Hu P*, Peng S, Liu Y, Li W, Wang C, He S, Zhai W, Xu Q, Chen L (2017) Genetic Adaptation of Schizothoracine Fish to the Phased Uplifting of the Qinghai–Tibetan Plateau.G3: Genes|Genomes|Genetics7: 1267-1276.

[12] Zhang D,Hu P, Liu T, Wang J, Jiang S, Xu Q, Chen L (2018) GC bias lead to increased small amino acids and random coils of proteins in cold-water fishes. BMC Genomics19: 315.

[13] Zhou T, Gui L, Liu M, Li W,Hu P, Duarte DFC, Niu H, Chen L (2019) Transcriptomic responses to low temperature stress in the Nile tilapia, Oreochromis niloticus.Fish & Shellfish Immunology84: 1145-1156.

02 主要学术成就

1.首批开发了高通量单细胞新生转录本标记技术scNT-seq

2.第一批开发了高通量单细胞核转录组测序技术sNucDrop-seq

3.合作研发了单碱基分辨率的5-羟甲基胞嘧啶(5hmC)测序技术ACE-seq，对起始DNA需求量减少了1000倍，为研究稀缺样本基因组中胞嘧啶修饰提供了新的研究手段；进一步研发了单细胞单碱基分辨率的5hmC测序技术snhmC-seq。

4.首次解析了哺乳动物出生后心脏细胞的异质性

5.在小鼠脑垂体前叶中鉴定出了一种新的细胞类型，该成果以共同第一作者排第二在2020年发表于SCI刊物Protein & Cell

6.系统性地鉴定了鱼类基因组中低温响应的顺式作用元件，相关成果以第一作者发表在SCI刊物Nucleic Acids Research等杂志。

03 近期主要科研项目

1. 利用高通量单细胞多组学测序技术解析南极冰鱼血红细胞丢失的分子机制，国自然基金委，2023/1-2025/12

2. 单细胞多组学解析高温诱导罗非鱼性逆转的分子机制，国自然基金委，2022/10-2025/9

04 近期荣誉、获奖

鱼类抗冻蛋白基因的起源和低温适应的分子发育机制研究，2021年教育部高等学校科学研究优秀成果奖（科学技术）一等奖